Autoantigen Array

Price per array (15 samples): $1400.00

The “Autoantigen Microarray” bears 80 autoantigens and various internal controls. The autoantigens in this panel include most of nuclear antigens, cytoplasmic antigens, membrane antigen, phospholipid antigens, as well as some novel autoantigen commonly implicated in systemic and organ-specific autoimmune diseases. 

Sample types: Serum, Plasma, Lysates, Other fluids

Volume Required: 10 μL

Autoantibody Profiling Service:

Profiling IgG and IgM autoantibodies from serum or other body fluids. Minimum 10 ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with the autoantigen array. The autoantibodies binding to the antigens on the array will be detected using Cy3 labeled anti-IgG and Cy5 labeled anti-IgM and the arrays will be scanned with GenePix® 4300A Microarray Scanner. The images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgG or IgM). The final data output will an Excel spreadsheet listing non-normalized and Normalized signal intensity values for each autoantibody as well as the Signal to Noise Ratio (SNR) for all targets. (Note: Other Ig isotypes, or fluids from other species can also be screened, if requested).

The antigens covered by the array are listed below:

AggrecanElastinMDA5SAE1/SAE2 
Alanyl-tRNA Synthetase (PL-12)Fibrinogen Type I-SMi-2Sm
Asparaginyl-tRNA Synthetase (KS)Formiminotransferase Cyclodeaminase (LC1)Myelin basic protein (MBP)Sm/RNP
Bactericidal/permeability-increasing protein (BPI)Genomic DNAMyeloperoxidase (MPO)SmD
ß2-Glycoprotein 1 GliadinMyosinSmD1
BCOADC-E2Glomerular Basement Membrane (GBM)NucleolinSP100
CardiolipinGlutamate Decarboxylase 65 kDa (GAD65; ng)Nucleosome Fibrinogen IV
Centromere Protein A (CENP-A)GP2Nup 62SRP54
Centromere Protein B (CENP-B)GP210NXP2 also known as MORC3ssDNA
Chondroitin sulfateHeparan sulfate proteoglycan(HSPG)OGDC-E2T1F1 gamma
Collagen III  Histidyl-tRNA Synthetase (Jo-1)PDC-E2Threonyl-tRNA Synthetase (PL-7)
Collagen VHistonePM/Scl 100Thyroglobulin
Complement C3Human CD 40PM/Scl-75Thyroid Peroxidase (TPO)
Complement C4IA-2 (ICA 512)Insulinoma-associated protein (IA-2)Proliferating Cell Nuclear Antigen (PCNA)Tissue Transglutaminase (tTG)
Complement component C1q receptor (C1q)InsulinProteinase 3 (PR3)TNF-alpha
Cytochrome cIntrinsic Factor (IF)ProteoglycanU1-snRNP 68/70 kDa
Cytochrome P450 2D6 (LKM 1; ng)KU (P70/P80)Ribosomal Phosphoprotein P0U1-snRNP A
DFS70La/SS-BRibosomal Phosphoprotein P2U-snRNP B/B’
DNA Topoisomerase I (Scl-70)LamininRo/SS-A (52 kDa)Vimentin
Double-stranded DNA (dsDNA)M2Ro/SS-A (60 Kda)Vitronectin

The autoantigens are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control.