Autoantigen Array

Price per array (15 samples): $1350.00

The “Autoantigen Microarray” bears 128 autoantigens and various internal controls. The autoantigens in this panel include most of nuclear antigens, cytoplasmic antigens, membrane antigen, phospholipid antigens, as well as some novel autoantigen commonly implicated in systemic and organ-specific autoimmune diseases. 

Sample types: Serum, Plasma, Lysates, Other fluids

Volume Required: 10 μL

Autoantibody Profiling Service:

Profiling IgG and IgM autoantibodies from serum or other body fluids. Minimum 10 ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with the autoantigen array. The autoantibodies binding to the antigens on the array will be detected using Cy3 labeled anti-IgG and Cy5 labeled anti-IgM and the arrays will be scanned with GenePix® 4300A Microarray Scanner. The images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgG or IgM). The final data output will an Excel spreadsheet listing non-normalized and Normalized signal intensity values for each autoantibody as well as the Signal to Noise Ratio (SNR) for all targets. (Note: Other Ig isotypes, or fluids from other species can also be screened, if requested).

The antigens covered by the array are listed below:

ACE2 FACTOR H KS Prothrombin
Aggrecan FACTOR I KU (P70/P80) Ro/SS-A (52 kDa)
Alpha Fodrin FACTOR P La/SS-B Ro/SS-A (60 Kda)
Amyloid β 1-42 Fibrinogen Type I-S Laminin SAE1/SAE2
AQP4 Fibronectin LC1 Scl-70
BAFF GAD65 Liver Cytosol 1 Sm
ß2-Glycoprotein 1Genomic DNALPSSmD
CD40H/K-ATPaseMDA5T1F1 gamma
CENP-BHistone H1MitochondrionTLR4
Chondroitin sulfateHistone H2AMPOTNF-alpha
Collagen IIHistone H2BMyosinTPO
Collagen IIIHistone H3Nrp1Troponin I
Collagen IVHSPGNucleolintTG
Complement C1qIA-2Nucleosome

U1-snRNP A

Complement C3IFNup 62

U-snRNP B/B’

Complement C4IFN-αNXP2

U1-snRNP C

Complement C5IFN-βOGDC-E2


Complement C6IFN-γP0


Complement C7IFN-λ1P2

HIgControl 1

Complement C8IL-12/NKSFPCNA

HIgControl 2

Complement C9IL-15PDC-E2

HIgControl 3


HIgControl 4

Cytochrome cIL-6PL-7

MIgControl 1

DFS70INF-εPM/Scl 100

MIgControl 2


MIgControl 3


MIgControl 4


The autoantigens are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control.