Price per array (15 samples): $1350.00
The “Exposome Microarray” bears 72 environmental antigens including 53 viral antigens and 12 environmental allergens, together with various internal controls.
Sample types: Serum, Plasma, Lysates, Other fluids
Volume Required: 10 μL
Autoantibody Profiling Service:
IgG and IgM autoantibodies will be profiled from serum or other body fluids. A minimum of 10 ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be diluted 1:50, and incubated with the array. The antibodies binding to the antigens on the array will be detected using Cy3 labeled anti-IgG and Cy5 labeled anti-IgM and the arrays will be scanned with GenePix® 4300A Microarray Scanner. The images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each exposome antigen will be normalized to internal controls. The final data output will an Excel spreadsheet listing non-normalized and Normalized signal intensity values for each antibody as well as the Signal to Noise Ratio (SNR) for all targets. (Note: IgE, other Ig isotypes, or fluids from other species can also be screened, if requested).
The antigens covered by the array are listed below:
| Adenovirus (ADV type 5) natural antigen |
| CMV Ext-2, enriched for cell surface glycoprotein antigens |
| CMV III Antigen, enriched for pp65 |
| CMV-G, whole cell extract |
| CMV-M Concentrate, nuclear extract and ER antigens |
| Dengue virus (DENV) |
| EBNA1 |
| Echovirus type 9 |
| Enterovirus 71 VP1 (EV71) |
| H1N1 HA (?TM) (aa18-529, Strain A/Michigan/45/2015) |
| Haemophilus influenza OmpP2 |
| HCoV-229E S/40605 |
| HCoV-HKU1 S/40606 |
| HCoV-HKU1 S1/10312 |
| HCoV-NL63 Spike S1/2 |
| HCoV-OC43 S/40607 |
| Hepatitis A Virus (HAV) Antigen |
| Hepatitis A Virus (HAV) Antigen, concentrate |
| Hepatitis B surface antigen |
| Herpes simplex virus-1 (HSV-1) |
| Herpes simplex virus-2 (HSV-2) |
| Inactivated Respiratory Syncytial Virus Antigen |
| Influenza A antigen |
| Influenza B antigen |
| MERS-CoV NP/40068-V08B |
| MERS-CoV S/40069 |
| Molluscum Contagiosum |
| Mumps virus antigen Inactivated |
| Native cytomegalovirus |
| Native Influenza A Virus H3N2 |
| Native Influenza B Virus Antigen |
| Norovirus vp1 |
| Parvovirus VP1 (aa 1-781) |
| Respiratory Syncytial Virus (RSV) long strain |
| ROTAVIRUS SA-11 ANTIGEN |
| RUBELLA GRADE IV ANTIGEN PBS |
| Rubella RSVP Antigen |
| RUBELLA VIRUS GRADE III ANTIGEN |
| RUBEOLA ANTIGEN |
| SARS-CoV-1 NP/40143-V08B |
| SARS-CoV-1 S1 RBD/40150-V08B2 |
| SARS-CoV-1 S1/40150-V08B1 |
| SARS-CoV-2 NCP/094 |
| SARS-CoV-2 NCP/40588 |
| SARS-CoV-2 S1 RBD/40592 |
| SARS-CoV-2 S1/091 |
| SARS-CoV-2 S1/40591 |
| SARS-CoV-2 S1+S2/40589 |
| SARS-CoV-2 S2 ECD/40590 |
| SARS-CoV-2_S1_RBD(F342L) |
| Varicella Zoster Virus (VZV) Antigen |
| Varicella Zoster Virus (VZV) II Antigen |
| West Nile Virus (WNV) Envelope protein |
| Diphtheria Toxin |
| M. Tuberculosis CFP10 |
| M. Tuberculosis ESAT-6 Antigen |
| Mycoplasma Pneumoniae Antigen |
| Pseudomonas fluorescense I2 |
| Streptococcus pneumoniae |
| TETANUS TOXIN |
| Toxoplasma Antigen (Toxo) grade II antigen |
| Bermuda_Cynodon dactylon (Bermouda grass) |
| Cat dander |
| Cedar,Red_Juniper rus virginiana |
| Cockroach |
| Coxsackievirus (B1) Native Antigen |
| Dog Dander, Mixed-Breed_Canis familiaris |
| Dog Epithelia, Mixed-Breed_Canis familiaris |
| Honey Bee_Apis mellifera |
| House Dust |
| Mite, House Dust_Blomia tropicalis |
| Wheat, Whole_Triticum aestivum |