The “Autoantigen Microarray” bears 128 autoantigens and various internal controls. The autoantigens in this panel include most of nuclear antigens, cytoplasmic antigens, membrane antigen, phospholipid antigens, as well as some novel autoantigen commonly implicated in systemic and organ-specific autoimmune diseases. 

Sample types: Serum, Plasma, Lysates, Other fluids

Volume Required: 10 μL

Autoantibody Profiling Service:

Profiling IgG and IgM autoantibodies from serum or other body fluids. Minimum 10 ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with the autoantigen array. The autoantibodies binding to the antigens on the array will be detected using Cy3 labeled anti-IgG and Cy5 labeled anti-IgM and the arrays will be scanned with GenePix® 4300A Microarray Scanner. The images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgG or IgM). The final data output will an Excel spreadsheet listing non-normalized and Normalized signal intensity values for each autoantibody as well as the Signal to Noise Ratio (SNR) for all targets. (Note: Other Ig isotypes, or fluids from other species can also be screened, if requested).

The antigens covered by the array are listed below: